Journal: International Journal of Molecular Sciences

Article Title: Enhanced Anti-Cancer Effects of Conditioned Medium from Hypoxic Human Adult Dermal Fibroblasts on Cervical Cancer Cells

doi: 10.3390/ijms23095134

Figure Lengend Snippet: Nodes and edges in the PPIs of intracellular proteins up- and down-regulated (> 2-fold) in HeLa cells treated with H-CM compared with N-CM.

Article Snippet: The membranes were blocked with 4% skim milk for 2 h at room temperature and then incubated with rabbit anti-human CD19 (1:3000 dilution; cat. no. CSB-RA780821A0HU; CUSABIO, Wuhan, China), CHEK1 (1:3000 dilution; cat. no. CSB-RA176809A0HU; CUSABIO), ESR1 (1:3000 dilution; cat. no. CSB-PA11399A0Rb; CUSABIO), LCK (1:3000 dilution; cat. no. CSB-PA009798; CUSABIO), MCL1 (1:3000 dilution; cat. no. CSB-PA03829A0Rb; CUSABIO), PCNA (1:3000 dilution; cat. no. CSB-PA-208009; CUSABIO), POLA1 (1:3000 dilution; cat. no. CSB-PA002170; CUSABIO), TBP (1:3000 dilution; cat. no. CSB-RA821481A0HU; CUSABIO), or TNF antibody (1:3000 dilution; cat. no. CSB-PA07427A0Rb; CUSABIO) and mouse anti-human ꞵ-actin antibody (1:10,000 dilution; cat. no. A1978; Sigma) overnight at −4 °C.

Techniques:

Journal: Translational Pediatrics

Article Title: Single-cell transcriptomics and network pharmacology reveal therapeutic targets of Jianpi Yiqi Bugan Yishen decoction in immune cell subsets of children with myasthenia gravis

doi: 10.21037/tp-22-593

Figure Lengend Snippet: Compound docking with the target protein molecule. The yellow dotted line shows hydrogen bonding, and the green line shows amino acids that form hydrogen bonds with the compound. (A) STAT1—Quercetin. (B) JUN—Quercetin. (C) CXCL10—Quercetin. (D) ADRB2—Quercetin. (E) FOS—Quercetin. (F) JUN—Luteolin. (G) HSP90AA1—Luteolin. (H) MCL1—Luteolin. (I) ADRB2—Resveratrol.

Article Snippet: 1), MCL1 (HUABIO, Cat. ET1606-14), and c-FOS (HUABIO, Cat. ET1701-95) immunohistochemical staining was performed on the muscle tissue of the rats.

Techniques:

Journal: Translational Pediatrics

Article Title: Single-cell transcriptomics and network pharmacology reveal therapeutic targets of Jianpi Yiqi Bugan Yishen decoction in immune cell subsets of children with myasthenia gravis

doi: 10.21037/tp-22-593

Figure Lengend Snippet: Components docked with key targets

Article Snippet: 1), MCL1 (HUABIO, Cat. ET1606-14), and c-FOS (HUABIO, Cat. ET1701-95) immunohistochemical staining was performed on the muscle tissue of the rats.

Techniques: Binding Assay

Journal: British Journal of Cancer

Article Title: A direct comparison of selective BH3-mimetics reveals BCL-X L , BCL-2 and MCL-1 as promising therapeutic targets in neuroblastoma

doi: 10.1038/s41416-020-0795-9

Figure Lengend Snippet: a Expression of BCL-2 proteins in neuroblastoma cell lines was assessed by Western blotting. One representative experiment is shown ( n = 3). GAPDH was used as a loading control. b For quantification, three independent experiments were analysed by densitometry to calculate mean expression of BCL-2 family proteins versus loading control. Data are shown as a heatmap. c Linear regression analysis demonstrates a correlation between BCL-2 expression and sensitivity to ABT-199, as assessed by calculation of EC 50 values based on the CellTiterGlo data presented in Fig. . Linear regression analysis indicates no significant correlation between BCL-X L levels and sensitivity to A1331852 or MCL-1 levels and sensitivity to S63845. n.s. Not significant.

Article Snippet: Western blotting was performed using the following antibodies: mouse anti-BCL-2 (Dako, M088701-2, Hamburg, Germany), rabbit anti-BCL-X L (Cell Signaling, 2762S, Beverly, MA), rabbit anti-MCL-1 (Enzo, ADI-AAP-240F, Farmindale, NY), rabbit anti-BCL-w (Cell Signaling, 2724S), rabbit anti-BIM (Cell Signaling, 3183S), mouse anti-NOXA (Enzo, ALX-804-408), rat anti-BMF (Enzo, ALX-804-343), rabbit anti-BAK (Upstate/Merck, 06-536), mouse anti-BAX (BD Bioscience, 610983), rabbit anti-PUMA (Cell Signaling, 4976S), rabbit anti-caspase-3 (Cell Signaling, 9662S), mouse anti-PARP (poly(ADD)ribose polymerase) (Cell Signaling, 9546S), mouse anti-β-actin (Sigma, A5441, Deisenhofen, Germany) or mouse anti-GAPDH (glyceraldehyde 3-phosphate dehydrogenase) (BioTrend, 5G4-6C5).

Techniques: Expressing, Western Blot

Journal: British Journal of Cancer

Article Title: A direct comparison of selective BH3-mimetics reveals BCL-X L , BCL-2 and MCL-1 as promising therapeutic targets in neuroblastoma

doi: 10.1038/s41416-020-0795-9

Figure Lengend Snippet: a IMR-32 cells were treated with A1331852 (1 μM) and Kelly cells were treated with S63845 (1 μM) for 4 h before lysis in CHAPS buffer. Interaction of pro- and antiapoptotic BCL-2 proteins was investigated by IP of antiapoptotic BCL-2 proteins MCL-1, BCL-X L or BCL-2 upon treatment with BH3-mimetics. b IMR-32 cells were treated with 1 μM A1331852 (upper panel) and Kelly cells were treated with 1 μM S63845 (lower panel) for 4 h before lysis in CHAPS buffer. Interaction of BAK with antiapoptotic BCL-2 proteins was investigated by IP with anti-BAK antibody. GAPDH is shown as a loading control.

Article Snippet: Western blotting was performed using the following antibodies: mouse anti-BCL-2 (Dako, M088701-2, Hamburg, Germany), rabbit anti-BCL-X L (Cell Signaling, 2762S, Beverly, MA), rabbit anti-MCL-1 (Enzo, ADI-AAP-240F, Farmindale, NY), rabbit anti-BCL-w (Cell Signaling, 2724S), rabbit anti-BIM (Cell Signaling, 3183S), mouse anti-NOXA (Enzo, ALX-804-408), rat anti-BMF (Enzo, ALX-804-343), rabbit anti-BAK (Upstate/Merck, 06-536), mouse anti-BAX (BD Bioscience, 610983), rabbit anti-PUMA (Cell Signaling, 4976S), rabbit anti-caspase-3 (Cell Signaling, 9662S), mouse anti-PARP (poly(ADD)ribose polymerase) (Cell Signaling, 9546S), mouse anti-β-actin (Sigma, A5441, Deisenhofen, Germany) or mouse anti-GAPDH (glyceraldehyde 3-phosphate dehydrogenase) (BioTrend, 5G4-6C5).

Techniques: Lysis